Wayne State University recently received a five-year, $1.925 million grant from the National Eye Institute of the National Institutes of Health to test the role of microRNAs (miRNAs) — a newly recognized level of gene expression regulation — in bacterial keratitis, an infection of the cornea caused by bacteria, and to identify new therapeutic targets and alternative treatment strategies.
According to the research team led by Shunbin Xu, M.D., Ph.D., associate professor of Ophthalmology and of Anatomy and Cell Biology in the School of Medicine at Wayne State University, Pseudomonas aeruginosa (PA)-induced keratitis is one of the most rapidly developing and destructive diseases of the cornea and a global cause of visual impairment and blindness. It is also one of the most causative organisms of contact lens-related diseases in developed countries.
Dr. Xu, along with Linda Hazlett, Ph.D., interim vice dean for Research and Graduate Programs in Wayne State’s School of Medicine and chair of the Department of Anatomy and Cell Biology, will test the role of the miR-183/96/182 cluster and the mechanisms by which it regulates the innate immune response to PA infection, as well as the potential of knockdown of the cluster function by anti-miRs to protect the cornea from adverse effects of the disease.
“In our previous studies, we discovered that anti-miR-183/96/182 enhances the ability of innate immune cells — including macrophages and neutrophils from our blood — to engulf and kill bacteria,” Dr. Xu said. “This also limits inflammatory responses and collateral damage to the corneal tissue.
“Through our research, we hope that novel therapies will be developed for treatment of corneal bacterial infections in contact lens users, as well as injured soldiers on the battlefield,” he said.
The goal of this grant, “The miR-183/96/182 Cluster in Pseudomonas Aeruginosa-Induced Keratitis,” is to uncover the underlying molecular mechanisms by which the cluster enhances the innate immunity, which may lead to development of new approaches to combat antibiotic-resistant bacterial infections and tumors evading the surveillance of the immune system.
The award number for this National Eye Institute of the National Institutes of Health grant is EY026059.