Department of Pharmacology

Chair: Bonnie Sloane, PhD
6374 Scott Hall
(313) 577-1580

Articles, Patents and Intellectual Properties

Elhamdani A, Martin TFJ, Kowalchyk JA, Artalejo CR.  CAPS (Ca2+-dependent activator protein for secretion) is critical for the fusion of dense-core vesicles with the membrane in calf adrenal chromaffin cells.  J Neurosci 19(17):7375-7383, 1999.

Elhamdani A, Brown ME, Artalejo CR and Palfrey HC.  Enhancement of the dense-core vesicle secretory cycle by glucocorticoid differentiation of PC12 cells: characteristics of rapid exocytosis and endocytosis.  J Neuroscience, in press.

Bjarnadottir M., Wulff B, Sameni M, Sloane BF, Keppler D, Grubb A,  Abrahamson M. Intracellular accumulation of the amyloidogenic L68Q variant of human cystatin C in NIH/3T3 cells.  Mol. Pathol. 51: 317-326, 1998.

Keppler D, Walter R, Sierra F.  Increased expression of mature cathepsin B in aging rat liver.  Cell & Tissue Res accepted pending revisions.

Linebaugh BE, Sameni M, Day NA, Sloane BF, Keppler, D.   Exocytosis of active cathepsin B: enzyme activity at pH 7.0, inhibition and molecular mass.  Eur J Biochem 263:100-109, 1999.

Kessel D, Luo Y.  Photodynamic therapy:  a mitochondrial inducer of apoptosis.  Cell Death Differ 6: 28-35, 1999.

Kessel D, Dougherty TJ.  Agents used in photodynamic therapy.  Rev. Comtemp Pharmacother 10:19-24, 1999.

Amit I, Malik Z, Kessel D.  Photoproduct formation from a zinc benzochlorin iminium salt detected by fluorescence microscopy.  Phytochem Photobiol 69: 700-702, 1999.

Zalups RK, Barfuss DW, Lash LH. Disposition of inorganic mercury following biliary obstruction and chemically-induced glutathione depletion:  Dispositional changes one hour after the intravenous administration of mercuric chloride. Toxicol Appl Pharmacol 154:135-144, 1999.

Lash LH, Putt DA, Abbas R, Brashear WT, Parker JC, Fisher JW. Identification of S-(1,2-dichlorovinyl)glutathione in the blood of human volunteers exposed to trichloroethylene. J Toxicol Environ Health 56:1-21, 1999.

Lash LH, Lipscomb JC, Putt DA, Parker JC. Glutathione conjugation of Trichloroethylene in human liver and kidney:  Kinetics and individual variation. Drug Metab Dispos 27:351-359, 1999.

Lash LH, Putt DA. Renal cellular transport of exogenous glutathione:  Heterogeneity at physiological and pharmacological concentrations. Biochem Pharmacol 58:897-907, 1999.

Cummings BS, Zangar RC, Novak RF, Lash LH. Cellular distribution of cytochromes P-450 in the rat kidney.  Drug Metab Dispos 27:542-548, 1999.

Lash LH, Putt DA, Zalups RK. Influence of exogenous thiols on mercury-induced cellular injury in isolated renal proximal tubular and distal tubular cells from normal and uninephrectomized rats.  J Pharmacol Exp Ther 291:492-502, 1999.

Zalups RK, Barfuss DW, Lash LH. Relationships between alterations in glutathione metabolism and the disposition of inorganic mercury in rats:  Effects of biliary ligation and chemically-induced modulation of glutathione status. Chem-Biol Interact 123:171-195, 1999.

Chen Z, Putt DA, Lash LH. Enrichment and functional reconstitution of glutathione transport activity from rabbit kidney mitochondria:  Further evidence for the role of the dicarboxylate and 2-oxoglutarate carriers in mitochondrial glutathione transport. Arch Biochem Biophys, in press.

Cummings BS, Parker JC, Lash LH. Role of cytochrome P450 and glutathione S-transferase a in metabolism and cytotoxicity of trichloroethylene in rat kidney. Biochem Pharmacol, in press.

Cummings BS, Lash LH. Metabolism and toxicity of trichloroethylene and S-(1,2-dichlorovinyl)-L-cysteine in freshly isolated human proximal tubular cells. Toxicol Sci, in press.

Wong SC, Zhang  L, Witt  TL, Proefke  SA, Bhushan  A, and Matherly  LH. Impaired methotrexate transport in methotrexate resistant CCRF-CEM cells involves early translation termination and increased turnover of a mutant reduced folate carrier.  J Biol Chem 274: 10388-10394, 1999.

Taub JW, Huang X, Matherly LH, Stout ML, Buck SA, Massey GV, Becton DL, Chang MN, Weinstein, HJ, Ravindranath Y. Expression of chromosome-21 localized genes in acute myeloid leukemia: Differences between down syndrome and non-down syndrome blast cells and relationship to In Vitro sensitivity to cytosine arabinoside and daunorubicin, Blood  99:1393-1400, 1999.

Matherly LH, Taub JW.  Molecular and cellular correlates of methotrexate response in childhood acute lymphoblastic leukemia, Leukemia and Lymphoma, 35: 1-20, 1999.

Mattingly RR, Saini, V, Macara, IG.  Activation of the Ras-grf/CDC25Mm exchange factor by lysophosphatidic acid.  Cellular Signalling, 11:603-610, 1999.

Mattingly RR.  Phosphorylation of Serine-916 of ras GRF1 contributes to the activation of exchange factor activity by muscarinic receptors.  J Biol Chem, 274:37379-37384, 1999.

Angeles R, Devine J, Barton  K, Smith  M, McCauley, R.  Mutation of K234 and K236 in the voltage dependent anion channel 1 impair its insertion into the mitochondrial outer membrane.  J Bioenerg Biomemb 31:137-142, 1999.

Angeles R, Devine J, Barret R, Goebel D, Blachly-Dyson E,  Forte M,  McCauley R.  Mutations in the voltage dependent anion channel of the mitochondrial outer membrane cause of dominant non-lethal growth impairment.  J Bioenerg Biomemb 31:143-151, 1999.

Schneider DR.  Method and apparatus for preserving human saliva for testing.  PCT Filed, 11/98.

Schneider DR.  Method of apparatus for preserving human saliva for testing US Patent approved 2/99.

Schneider DR.  Method and apparatus for preserving human saliva for testing.  PCT Published, 6/99.

Schneider DR.  Method and apparatus for preserving human saliva for testing.  CIP-1 filed, 9/99.

Schneider DR.  Method and apparatus for preserving human saliva for testing.  Patent issued, US Patent #5,968,746, 10/99.

Schneider DR.  Method and apparatus for preserving human saliva for testing.  CIP-2 filed, 1/00.

Schubiner H, Tzelepis A, Millberger F, Lockhart N, Kruger M, Kelley B, Schoener EP.  Prevalence of attention deficit hyperactivity and conduct disorder among substance abusers.  J Clinical Psychiatry.  In press.

Nishimura Y, Sameni M, and Sloane BF.  Malignant transformation alters intracellular trafficking of lysosomal cathepsin D in human breast epithelial cells.  Pathol Oncol Res 4: 283-296, 1998.  [Citation not available for 1998 annual report]

Linebaugh BE, Sameni M, Day NA, Sloane BF, Keppler D.  Exocytosis of active cathepsin B:  Enzyme activity at pH 7.0, inhibition and molecular mass.  Eur J Biochem 264: 100-109, 1999.

Berquin IM, Yan S-Q, Huang L, Sloane BF, Troen BR.  Differentiating agents regulate cathepsin B gene expression in HL-60 cells.  J Leukoc Biol 66: 609-616, 1999.

Demchik L, Sameni M, Nelson K, Rozhin J, Mikkelsen T, Sloane, BF.  Cathepsin B and glioma invasion.  Intl. J. Dev. Neurosci. 17: 483-494, 1999.

Sinha AA, Quast BJ, Korkowski JC, Wilson MJ, Reddy PK, Ewing SL, Sloane, BF, Gleason DF.  The relationship of cathepsin B and stefin A mRNA localization identifies a potentially aggressive variant of human prostate cancer within a Gleason histologic score.  Anticancer Res. 19: 2821-2830, 1999.

Yan S-Q, Berquin IM, Troen BR, Sloane, BF.  Transcription of human cathepsin B is mediated by Sp1 and Ets family factors in glioma.  DNA Cell Biol., in press.

Mai J, Finley R, Waisman DM, Sloane, BF.  Procathepsin B interacts with the annexin II tetramer on the surface of tumor cells.  J. Biol. Chem., in press.

Tisdale EJA. Rab2 mutant with impaired GTPase activity stimulates vesicle formation from pre-Golgi intermediates.  Mol Biol Cell 10:1837-1849, 1999.

Zhao Z, Crossland WJ, Kulkarni JS, Wakade TD, Wakade AR.  2Õ-Deoxyadenosine causes cell death in embryonic chicken sympathetic ganglia and brain. Cell Tissue Res :  296, 281-291. 1999.

Books, Chapters and Reviews

Shridhar R, Sloane BF, Keppler D.  Inhibitors of Papain-like Cysteine Peptidases in Cancer.  In:  Retinoids & Proteases as Targets for Therapy.  K. von der Helm, B.C. Korant and J.C. Cheronis, Eds.  Handbook of Experimental Pharmacology, Vol. 140, pp. 301-328, Springer Verlag, Berlin Heidelberg, 1999.

Lash LH. Measurement of glutathione transport. In: Current Protocols in Toxicology, (DJ Reed, ed), John Wiley & Sons, New York, section 6.3 (1999).

Lash LH, Fisher JW, Lipscomb JC, Parker JC. Metabolism of Trichloroethylene. Environ Health Perspec, in press (1999).

Lash LH, Parker JC, Scott CS. Modes of action of trichloroethylene for kidney tumorigenesis. Environ Health Perspec, in press (1999).

Lash LH. Role of glutathione in the metabolism, transport and toxicity of metals.  In: The Molecular Biology and Toxicology of Metals, (RK Zalups and DJ Koropatnick, eds), Taylor & Francis, London, in press.

Frosch BA, Berquin I, Emmert-Buck MR, Moin K, Sloane, BF. Molecular regulation, membrane association and secretion of tumor cathepsin B. APMIS 107: 28-37, 1999.

Moin K, Demchick L, Mai J, Duessing J, Peters J, Sloane BF.  Observing proteases in living cells.  In, cellular peptidases in immune functions and diseases (2).  in press.

Moin K, Demchick, L, Mai J, Duessing J, Peters J, Sloane BF. Observing proteases in living cells. In, Cellular peptidases in immune functions and diseases (2).  in press.

Frosch BA, Berquin IM, Emmert-Buck M, Moin K, Sloane, BF.  Molecular regulation, membrane association and secretion of tumor cathepsin B.  Acta Pathol. Microbiol. Immunol. Scandinavica 107: 28-37, 1999.

Demchik LL, Sloane BF.  Cell-surface proteases in cancer.  In:  Proteases: New Perspectives (V. Turk, ed.), Birkhauser Verlag, Basel, pp. 109-124, 1999.

Mai J, Waisman DM, Sloane, BF.  Cell surface complex of cathepsin B/annexin II tetramer in malignant progression.  Biophys Biochim Acta:  Protein Structure and Molecular Enzymology (in honor of Hans Neurath), in press.

Shridhar R, Sloane BF, Keppler D.  Inhibitors of papain-like cysteine peptidases in cancer.  In:  Proteases as Targets for Therapy, (K von der Helm, J Cheronis and B Korant, eds), Springer-Verlag, Heidelberg, in press.

Koblinski JE, Ahram M, Sloane, BF.  Unraveling the roles of proteases in cancer.  Clin Chim Acta, in press.

Moin K, Demchik LL, Mai J, Duessing J, Peters C, Sloane, BF.  Observing proteases in living cells.  Adv Exp Med Biol 477:  in press.

Shridhar R, Sloane BF, Keppler D.  Inhibitors of papain-like cysteine peptidases in cancer.  Handbook Exptl Pharmacol 140:301-328, 2000.

Yamazaki RK.  Chapter 6 Pharmacology. in Appleton & LangeÕs Review for the USMLE Step 1, 3rd Edition, (TK Barton, ed) Appleton & Lange, Stamford, CT, pp. 261-325, 2000.

Abstracts, Letters to Editors and Articles in Non-Refereed Journals

Cummings BS, Lasker JM, Lash LH. Expression of drug metabolism enzymes in human renal proximal tubular cells. Toxicol Sci 48 (1-S):96, 1999.

Putt DA, Lash LH, Zalups RK. Compensatory hypertrophy and cellular function and toxicity in primary cultures of rat renal cells. Toxicol Sci 48 (1-S):24, 1999.

Lash LH, Putt DA, Parker JC, Lipscomb JC. Kinetics and individual variation in GSH conjugation of trichloroethylene (Tri) in human liver and kidney. Toxicol Sci 48 (1-S):228, 1999.

Zalups RK, Barfuss DW, Lash LH. Effects of biliary ligation and modulation of GSH-status on the renal and hepatic disposition of inorganic mercury in rats. Toxicol Sci 48 (1-S):330, 1999.

Lash LH, Chen Z, Putt DA. Kinetics and substrate specificity of purified and reconstituted glutathione transport activity from rabbit kidney mitochondria. FASEB J 13:A62, 1999.

Lash LH, Putt DA, Zalups RK. Mitochondrial function after compensatory renal hypertrophy. FASEB J 13:A1039, 1999.

Thottassery J, Matherly LH, Valentine A, Relling MV, Evans WE, and Schuetz JD.  Functional inactivation of wild type p53 leads to selective antifolate sensitivity by purine nucleotide depletion.  American Association for Cancer Research 40: 291, 1999.

Zhang L, Ding BC, Witt T, Zhang Z, Hukku B, Matherly LH.  Human reduced folate carrier gene deletion and chromosomal translocations are causal elements in impaired membrane transport in methotrexate resistant K562 erythroleukemia cells. American Association for Cancer Research 40: 292, 1999.

Matherly LH, Taub JW, Zhang L. Zhang Z, Witt T, Hukku B, Shah S, Ravindranath Y.  Acute lymphoblastic leukemia cells with t(12;21) Exhibit increased levels and activity of human reduced folate carrier.  American Association for Cancer Research 40: 292, 1999.

Haber M, Gifford AJ, Matherly LH, Norris MD.  Transfection of the reduced folate carrier gene into methotrexate resistant cells alters their resistant phenotype.   American Association for Cancer Research 40: 675, 1999.

Taub JW, Ge Y, Huang X, Stout ML, Dutcher JA, Mohammad RM, Ravindranath Y, Matherly LH. Factors regulating expression of the chromosome 21-localized gene, cystathionine beta synthase, in down syndrome myeloblasts and its role in the high event-free survival rates of down syndrome patients with acute myeloid leukemia. Annual Meeting for American Society for Hematology: Blood 93: 382a, 1999.

Schoener EP, Scott RO.  Implementing evidence-based decision-making in a community mental health service system.  College on Problems of Drug Dependence, Proceedings, June, 1999.

Schoener EP, Dodammane S, Kardos S and Niedbala, RS.  Detection of abused drugs in oral fluid.  College on Problems of Drug Dependence, Proceedings, June 2000.

Grazier KL, Hegedus AM, Schoener EP.  Utilization of health services among substance abusing Medicaid women exposed to an integrated public-private managed care experiment.  College on Problems of Drug Dependence, Proceedings, June 2000.

Wakade TD, Wakade AR, Przywara DA Wakade TD.  Adenosine inhibits stimulation-evoked release of 3H-norepinephrine by intracellular action.  Neuroscience Abstracts, 25:1501, 1999.

Wakade AR, Przywara DA, Wakade TD.  Adenosine rescues chick embryo sympathetic neurons from NGF-withdrawal apoptosis by inhibiting caspases. Neuroscience Abstracts 25:550, 1999.