Established in 1993, my lab is interested in
the regulation and structure-function
relationships of contractile and
Muscle (cardiac, skeletal and smooth)
contraction and non-muscle cell motility
play vital roles in physiological activities
and pathological conditions. Our research is
focused on actin thin filament-associated
regulatory proteins: troponin and calponin,
to study their regulation and
structure-function relationships. Molecular
biology and genetic approaches are used to
investigate protein isoform evolution and
expression as well as to provide engineered
protein constructs for functional
characterization. Biochemical, biophysical
and immunochemical methods are used in
studying protein structure and function.
Cell culture systems and transgenic/gene
knock-out/knock-in mouse models are
developed for integrative functional
characterizations at cellular, organ and
whole animal levels.
Regulation and function of troponin T
Biochemical and biophysical studies are
performed to investigate the functional
significance of various troponin T isoforms
in the Ca2+-regulation of cardiac
and skeletal muscle contraction and
diseases. The studies are focused on the
abnormal cardiac troponin T splicing
variants found in dilated cardiomyopathy and
a slow skeletal muscle troponin T mutation
that causes a lethal type of nemaline
myopathy. This line of study is currently
funded by NIH grants R01HL078773 and
Post-translational regulation of troponin
during muscle adaptation and diseases:
Proteolytic modification of troponin I and
troponin T is studied for functional
significance and therapeutic values in the
Ca2+-regulation of myocardial
contraction in adaptation to hemodynamic
stresses, myocardial ischemia-reperfusion
injury, heart failure, and skeletal muscle
fatigue. This line of study is currently
funded by NIH grant R01HL098945 and a Group
Incubator Grant from WSU Office of Vice
President for Research.
Mechanical tension regulation and function
To study calponin's function as a troponin
analog in smooth muscle and non-muscle
cells, we are investigating their role in
the fine-tuning of smooth muscle
contractility, non-muscle motility, and the
function of actin cytoskeleton during
development, tissue remodeling and
cytokinesis. The study is focused on
mechanical tension regulated calponin gene
expression and protein degradation in
epithelial, endothelial, fibroblast,
macrophages and smooth muscle cells. This
line of study is currently funded by NIH
- (Last two years)
A complete list of Dr. Jin's publications can be
Troponin: Informative Diagnostic Marker.
Jin Ed., Nova Science Publishers, 2014
Wei H, Jin JP. N-terminal truncations of
cardiac troponin I and cardiac troponin T
produce distinct effects on contractility
and calcium homeostasis in adult
cardiomyocytes. Am J Physiol Cell Physiol.
2014 Dec 17. PMID: 25518962
Jin JP. Calponin: A mechanical tension-modulated regulator of
cytoskeleton and cell motility. Cur. Topics
in Biochem Res., (in press) 2014)
Hines PC, Gao X,
White JC, D'Agostino A, Jin
JP. A novel role of h2-calponin in
regulating whole blood thrombosis and
platelet adhesion during physiologic flow.
Physiol Rep. 2014 Dec 3;2(12). PMID:
Feng HZ, Chen G, Nan C, Huang X,
JP. Abnormal splicing in the N-terminal
variable region of cardiac troponin T
impairs systolic function of the heart with
preserved Frank-Starling compensation.
Physiol Rep. 2014 Sep 4;2(9). pii: e12139.
Moazzem Hossain M, Wang X, Bergan RC,
JP. Diminished expression of h2-calponin
in prostate cancer cells promotes cell
proliferation, migration and the dependence
of cell adhesion on substrate stiffness.
FEBS Open Bio. 2014 Jun 24;4:627-36. PMID:
Jin JP, Linke A, Walgenbach KJ, Rauch P, Zellmer A, Fimmers R, Kuhn
W, Hartmann G, Walgenbach-Brünagel G.
Calponin-h2: a potential serum marker for
the early detection of human breast cancer?
Tumour Biol. 2014 Nov;35(11):11121-7. PMID:
Corpeno R, Dworkin B, Cacciani N, Salah H,
Bergman HM, Ravara B, Vitadello M, Gorza L,
Gustafson AM, Hedström Y, Petersson J, Feng
HZ, Jin JP, Iwamoto H, Yagi N, Artemenko K,
Bergquist J, Larsson L. Time course analysis
of mechanical ventilation-induced diaphragm
contractile muscle dysfunction in the rat. J
Physiol. 2014 Sep 1;592(Pt 17):3859-80.
Wei H, Jin JP. A dominantly negative mutation
in cardiac troponin I at the interface with
troponin T causes early remodeling in
ventricular cardiomyocytes. Am J Physiol
Cell Physiol. 2014 Aug 15;307(4):C338-48.
Jin JP. Gene regulation, alternative splicing, and posttranslational
modification of troponin subunits in cardiac
development and adaptation: a focused
review. Front Physiol. 2014 Apr 30;5:165.
Liu R, Feng HZ,
Jin JP. Physiological contractility of cardiomyocytes in the wall of
mouse and rat azygos vein.
Am J Physiol Cell Physiol. 2014 Apr
1;306(7):C697-704. PMID: 24477237
Wei B, Lu Y,
Deficiency of slow skeletal muscle troponin
T causes atrophy of type I slow fibres and
decreases tolerance to fatigue. J Physiol.
2014 Mar 15;592(Pt 6):1367-80. PMID:
Huang QQ, Birkett R, Koessler RE, Cuda CM,
Haines GK 3rd,
JP, Perlman H, Pope RM.
Fas signaling in macrophages promotes
chronicity in K/BxN serum-induced arthritis.
Arthritis Rheumatol. 2014 Jan;66(1):68-77.
Zhang T, Choi SJ, Wang ZM, Birbrair A, Messi
ML, Jin JP, Marsh AP, Nicklas B, Delbono O.
Human slow troponin T (TNNT1) pre-mRNA
alternative splicing is an indicator of
skeletal muscle response to resistance
exercise in older adults. J Gerontol A Biol
Sci Med Sci. 2014 Dec;69(12):1437-47. PMID:
JP. NH2-terminal truncations of cardiac
troponin I and cardiac troponin T produce
distinct effects on contractility and
calcium homeostasis in adult cardiomyocytes.
Am J Physiol Cell Physiol. 2015 Mar
1;308(5):C397-404. PMID: 25518962
Akhter S, Jin JP. Distinct conformational and functional effects of two
adjacent pathogenic mutations in cardiac
troponin I at the interface with troponin T.
FEBS Open Bio. 2015 Jan 13;5:64-75. PMID:
Jin JP. The N-terminal hypervariable region of muscle type isoforms
of troponin T differentially modulates the
affinity of tropomyosin-binding site 1.
Biochemistry. 2015 Jun 10. PMID: 26024675
Flemming A, Huang QQ,
Jin JP, Jumaa H, Herzog S. A Conditional Knockout Mouse Model
Reveals That Calponin-3 Is Dispensable for
2Early B Cell Development. PLoS One. 2015
Jun 5;10(6):e0128385. PMID: 26046660
Jin JP. The Use of Affinity Tags to Overcome Obstacles in
Recombinant Protein Expression and
Purification. Protein Pept Lett. 2015 Jul
28. PMID: 26216265