Jian-Ping Jin, Ph.D.
Professor and Chairman
Lab: 5344 Scott Hall
Office: 5374 Scott Hall
540 E. Canfield, Detroit, MI 48201
313-577-1520, FAX 313-577-5494
jjin@med.wayne.edu

Editors in Chief, Archives of Biochemistry and Biophysics

Established in 1993, my lab is interested in the regulation and structure-function relationships of contractile and cytoskeleton proteins. Muscle (cardiac, skeletal and smooth) contraction and non-muscle cell motility play vital roles in physiological activities and pathological conditions. Our research is focused on actin thin filament-associated regulatory proteins: troponin and calponin, to study their regulation and structure-function relationships. Molecular biology and genetic approaches are used to investigate protein isoform evolution and expression as well as to provide engineered protein constructs for functional characterization. Biochemical, biophysical and immunochemical methods are used in studying protein structure and function. Cell culture systems and transgenic/gene knock-out/knock-in mouse models are developed for integrative functional characterizations at cellular, organ and whole animal levels.

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ONGOING PROJECTS:
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Regulation and function of troponin T isoforms: Biochemical and biophysical studies are performed to investigate the functional significance of various troponin T isoforms in the Ca2+-regulation of cardiac and skeletal muscle contraction and diseases. The studies are focused on the abnormal cardiac troponin T splicing variants found in dilated cardiomyopathy and a slow skeletal muscle troponin T mutation that causes a lethal type of nemaline myopathy. This line of study is currently funded by NIH grants R01HL078773 and R01AR048816.

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Post-translational regulation of troponin during muscle adaptation and diseases: Proteolytic modification of troponin I and troponin T is studied for functional significance and therapeutic values in the Ca2+-regulation of myocardial contraction in adaptation to hemodynamic stresses, myocardial ischemia-reperfusion injury, heart failure, and skeletal muscle fatigue. This line of study is currently funded by NIH grant R01HL098945 and a Group Incubator Grant from WSU Office of Vice President for Research.

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Mechanical tension regulation and function of calponin: To study calponin's function as a troponin analog in smooth muscle and non-muscle cells, we are investigating their role in the fine-tuning of smooth muscle contractility, non-muscle motility, and the function of actin cytoskeleton during development, tissue remodeling and cytokinesis. The study is focused on mechanical tension regulated calponin gene expression and protein degradation in epithelial, endothelial, fibroblast, macrophages and smooth muscle cells. This line of study is currently funded by NIH grant R01HL086720.

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SELECTED PUBLICATIONS - A complete list of Dr. Jin's publications can be found at PubMed-Jin
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Barbato, J.C., Huang, Q.-Q., Hossain, M.M., Bond, M., and Jin, J.-P.  (2005) Proteolytic N-Terminal Truncation of Cardiac Troponin I Facilitates Ventricular Relaxation and Enhances Heart Function. J. Biol. Chem. 280:6602-6609.

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Wang, X., Huang, Q.-Q. Breckenridge, M.T., Chen, A., Crawford, T.O., Morton, D.H., and Jin, J.-P. (2005) Cellular fate of truncated slow skeletal muscle troponin T produced by Glu180 nonsense mutation in Amish nemaline myopathy. J. Biol. Chem. 280:13241-13249.

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Hossain, M.M., Crish, J.F., Eckert R.L., Lin, J.J.-C., and Jin, J.-P. (2005) H2-calponin is regulated by mechanical tension and modifies the function of actin cytoskeleton. J. Biol. Chem. 280:42442-53.

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Brotto, M.A.P. Biesiadecki, B.J. Brotto, L.S., Nosek T.M. and. Jin, J.-P. (2006) Coupled Expression of Troponin T and Troponin I Isoforms in Single Skeletal Muscle Fibers Correlating to Contractility. Am. J. Physiol. Cell Physiol. 290:C567-C576.

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Zhang, Z., Biesiadecki, B.J., and Jin, J.-P. (2006) Selective removal of the N-terminal variable region of cardiac troponin T in ischemia-reperfusion by myofibril-associated m-calpain cleavage. Biochemistry 45:11681-94.

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Hossain, M.M., Smith, P.G., Wu, K., and Jin, J.-P. (2006) Cytoskeletal tension regulates both expression and degradation of h2-calponin in lung alveolar cells. Biochemistry 45:15670-83.

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Yu, Z.-B., Gao, F., Feng, H., and Jin, J.-P. (2007) Differential regulation of myofilament protein isoforms underlying the contractility changes in skeletal muscle unloading. Am. J. Physiol.:Cell Physiol., 292:C1192-203.

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Biesiadecki, B., Chong, S.M., Nosek T.M., and Jin, J.-P. (2007) Troponin T Core Structure and the Regulatory NH2-Terminal Variable Region. Biochemistry 46:1368-1379.

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Jin, J.-P., Zhang, Z., and Bautista, J.A. (2007) Isoform diversity, regulation and functional adaptations of troponin and calponin. Crit. Rev. in Eukar Gene Expr. 18:93-124 (invited review).

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Huang, Q.-Q. Feng, H.-Z., Liu, J., Du, J., Stull, L.B., Moravec, C., Huang, X. and Jin, J.-P. (2008) Co-expression of skeletal and cardiac troponin T decreases mouse cardiac function. Am J, Physiol.:Cell Physiol. 294:C213-222.

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Feng, H.-Z., Biesiadecki, B.J., Yu, Z.-B., Hossain, M.M., and Jin, J.-P. (2008) Restricted N-terminal truncation of cardiac troponin T: A novel mechanism for functional adaptation to energetic crisis. J. Physiol. (London) 586:3537-3550.

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Wu, K., and Jin, J.-P. (2008) Calponin in non-muscle cells. Cell Biochem. Biophys. 52:139-148. (invited review)

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Larsson,, L., Wang, X., Yu, F., Höök, P., Borg, K., Chong, SM, and Jin J.-P. (2008) Adaptation by alternative RNA splicing of slow troponin T isoforms in type 1 but not type 2 Charcot-Marie-Tooth disease. Am. J. Physiol. Cell Physiol. 295:C722-31.

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Huang, Q.-Q., Hossain, M.M., Wu, K., Parai., K., Pope, R.M., and Jin, J.-P. (2008) Role of h2-calponin in regulating macrophage motility and phagocytosis. J. Biol. Chem. 283:25887-99.

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Feng, H.-Z., Chen, M., Weinstein, L.S. and Jin, J.-P. (2008) Removal of the N-terminal extension of cardiac troponin I as a functional compensation for myocardial b-adrenergic deficiency. J. Biol. Chem. 283:33384-93.

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Chong, S.M., and Jin, J.-P. (2009) To Investigate Protein Evolution by Detecting Suppressed Epitope Structures. J. Mol. Evol. 68:448-60.

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Jeong, E., Wang, X., Xu, K., Hossain, M.M., and Jin, J.-P. (2009) Non-myofilament-associated troponin T fragments induce apoptosis. Am. J. Physiol. Heart Circ. Physiol. 297:H283-92.

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Feng, H.-Z., Wei, B., and Jin, J.-P. (2009) Deletion of a genomic segment containing the cardiac troponin I gene knocked down the expression of slow troponin T gene and impaired fatigue tolerance of diaphragm muscle. J. Biol. Chem. 284:31798–31806.

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Feng, H.-Z. and Jin, J.-P. (2010) Non-uniformity of Cardiac Troponin T Impairs Heart Efficiency. Am. J. Physiol. Heart Circ. Physiol. 299:H97-H105

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Wei, B., Gao, J., Huang, X.-P., and Jin, J.-P. (2010) Mutual rescues between two dominant-negative mutations in cardiac troponin I and cardiac troponin T. J. Biol. Chem. 285:27806-16.

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Wei, B., and Jin, J.-P. (2011) Troponin T Isoforms and Posttranscriptional Modifications: Evolution, Regulation and Function. Arch Biochem Biophy. 505:144-154. (invited review)

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Feng, H.-Z., Chen, M., Weinstein, L.S., and Jin, J.-P. (2011) Fast-to-slow fiber type switch increases fatigue resistance as a compensatory adaptation in Gsa-deficient soleus muscle. J Appl. Physiol. 111:834-843.

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Zhang, Z., Akhter, S., Mottl S., and Jin, J.-P. (2011) Ca2+-regulated conformational change in the COOH terminus of troponin I and binding to tropomyosin. FEBS J. 278:3348-3359.

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Zhang, Z., Feng, H.-Z., Jin, J.-P. (2011) Structure of the NH(2)-terminal variable region of cardiac troponin T determines its sensitivity to restrictive cleavage in pathophysiological adaptation. Arch Biochem Biophys. 515:37-45.

 

Dr. Jin's Photo Galleries:
    (Please click on the thumbnails below for an expanded view)

Lab Personnel


Lab Staff

Down time

Hard at work

Symposium

Fall 2013 Cardiology Conference
 Cordoba, Argentina
soccer game after the conference

Dr. Jin with Javier and his son.
Cordoba Argentina
Research

Anatomy of Actin Thin Filament Regulatory System

Regulation of Troponin & Calcitonin

The N-Terminal Region
Regulates TnT Conformation
& Function

Evolution Linkage
of TnT & Tnl
  
Enhanced Phagpcytosis of
Neurophils & Macrophages
  
Books

Click on image to enlarge
link to publisher

Release Date: June 15, 2014
 

last update: 04/15/2014 by CRC.