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Raymond F. Novak, Ph.D. ProfessorDirector, Institute of Environmental Health Sciences EHS Center in Molecular and Cellular Toxicology with Human Applications Institute of Environmental Health Sciences |
RESEARCH INTERESTS:
Our research focuses on the role of diabetes in altering the expression of cytochromes P450 (CYP2E1) and other enzymes involved in xenobiotic metabolism, oxidative stress and cellular protection in primary cultured hepatocytes at the level of transcription and translation, and the signaling pathways and components involved in regulating expression. Global gene expression is accomplished using microarray analysis and expression profiles, gene ontologies and signaling pathway analysis is performed in conjunction with intracellular signaling events.
Our laboratory also examines the effects of altered cell signaling on human breast cancer development. The ability of various endogenous and exogenous compounds and signaling components which alter the association of cell adhesion molecules that ultimately affect cell morphology, as well as cell cycle regulation, proliferation and apoptosis, is being studied. The various protein and gene markers that contribute to the development of Proliferative Breast Disease (PBD), a risk factor for tumorigenesis, is being examined using proteomic and microarray gene expression technologies.
Select Publications:
L.C. Overton, A. Hudder, and R.F. Novak (2007) “CYP2E1: Distribution, Xenobiotic Metabolism, and Regulation of Gene and Protein Expression: Role of Intracellular Signaling,” In: Cytochrome P450: Role in the Metabolism and Toxicity of Drugs and Other Xenobiotics (ed. C. Ionnides) Issues in Toxicology, Royal Society of Chemistry, London, UK., In Press.
Hudder A, Novak RF. miRNAs: effectors of environmental influences on gene expression and disease. Toxcol. Sci. 2008, Feb 16; [Epub ahead of print] In Press.
Kim SK, Novak RF. The role of intracellular signaling in insulin-mediated regulation of drug metabolizing enzyme gene and protein expression. Pharmacol Ther. 2007 Jan;113(1):88-120. PMID: 17097148
Kim SK, Abdelmegeed MA, Novak RF. The mitogen-activated protein kinase kinase (mek) inhibitor PD98059 elevates primary cultured rat hepatocyte glutathione levels independent of inhibiting mek.
Drug Metab Dispos. 2006 Apr;34(4):683-9.PMID: 16443668.
Dombkowski AA, Cukovic D, Novak RF. Secretome analysis of microarray data reveals extracellular events associated with proliferative potential in a cell line model of breast disease.
Cancer Lett. 2006 Sep 8;241(1):49-58. Epub 2005 Nov 17. PMID: 16298039
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