R. Daniel Bonfil, PhD
Associate Professor, Urology and Pathology

Education:
University of Buenos Aires, Buenos Aires, Argentina: M.Sc. (Licentiate) in Biological Sciences, 1981
University of Buenos Aires, Buenos Aires, Argentina: Ph.D., 1986
Department of Biological Sciences, Tumor Biology

Postdoctoral Training:
Postdoctoral Associate, Department of Pathology, Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA, 1986-1988

Professional and Faculty Appointments:
Head, Experimental Oncology Section, National Academy of Medicine, Buenos Aires, Argentina, 1989-1993
Investigator with Tenure of the Research Career of CONICET (National Council of Scientific and Technologic Research), Argentina, 1989-2002
Scientific Director, Fundación de Investigación del Cáncer, (Cancer Research Foundation), Buenos Aires, Argentina, 1991-2002
Head, Laboratory of Cancer Research at CEFYBO (Center for Pharmacological and Botanical Studies), Buenos Aires, Argentina, 1994-2002
Associate Professor, Wayne State University Departments of Urology and Pathology, 2002-Present
Full member, Proteases and Cancer Program, Barbara Ann Karmanos Cancer Institute, 2002-Present

Major Research Interests:
Tumor angiogenesis, tumor invasion and metastasis, tumor biology, biology of prostate cancer metastasis, matrix metalloproteinases

Selected Publications:

1. Bonfil, R.D., Ruggiero, R.A., Bustuoabad, O.D., Meiss, R.P., & Pasqualini, C.D. Role of concomitant resistance in the development of murine lung metastases. International Journal of Cancer 41: 415-422, 1988.
2. Bonfil, R.D., Reddel, R.R., Ura, H., Reich, R., Fridman, R., Harris, C.C., & Klein-Szanto, A.J.P. Invasive and metastatic potential of a v-Ha-ras transformed human bronchial epithelial cell line. Journal of the National Cancer Institute. 81: 587-594, 1989.
3. Ura, H., Nowak, S., Litwin, P., Watts, P., Bonfil, R.D., and Klein-Szanto, A.J.P. Effects of formaldehyde on normal xenotransplanted human tracheobronchial epithelium. American Journal of Pathology 134: 99-106, 1989.
4. Bonfil, R.D., Momiki, S., Conti, C.J., & Klein-Szanto, A.J.P. Benzoyl peroxide enhances the invasive ability of murine carcinoma cells. International Journal of Cancer 44: 165-169, 1989.
5. Ura, H., Bonfil, R.D., Reich, R., Reddel, R., Pfeifer, A., Harris, C.C., & Klein-Szanto, A.J.P. . Expression of type IV collagenase and procollagen genes and its correlation with the tumorigenic, invasive, and metastatic abilities of oncogene-transformed human bronchial epithelial cells. Cancer Research 49: 4615-4621, 1989.
6. Bonfil, R.D., Momiki, S., Fridman, R., Reich, R., Reddel, R., Harris, C.C., & Klein-Szanto, A.J.P. Enhancement of the invasive ability of a transformed human bronchial epithelial cell line by TPA and diacylglycerol. Carcinogenesis 10: 2335-2338, 1989.
7. Fridman, R., Lacal, J.C., Reich, R., Bonfil, R.D., & Ahn, C-H. Differential effects of phorbol ester on the in vitro invasiveness of malignant and non-malignant human fibroblast cells. Journal of Cell Physiology 142: 55-60, 1990.
8. Ruggiero, R.A., Bustuoabad, O.D., Cramer, P., Bonfil, R.D., & Pasqualini, C.D. Correlation between seric antitumor activity and concomitant resistance in mice bearing non-immunogenic tumors. Cancer Research 50: 7159-7165, 1990.
9. Bonfil, R.D., Medina, P., Gómez, D.E., Farías, E., Lazarowski, A, Meiss, R.P., & Bustuoabad, O.D. Expression of gelatinase/type IV collagenase in tumor necrosis correlates with cell detachment and tumor invasion. Clinical Experimental Metastasis 10: 211-220, 1992.
10. Bonfil, R.D., Vinyals, A., Bustuoabad, O.D., Llorens, A., Benavides, F.J., Gonzalez-Garrigues, M., & Fabra, A. Stimulation of angiogenesis as an explanation of Matrigel-enhanced tumorigenicity. International Journal of Cancer 58: 233-239, 1994.
11. Bonfil, R.D., Russo, D.M., & Schmilovich, A.J. Exposure to Vinorelbine inhibits in vitro proliferation and invasiveness of transitional cell bladder carcinoma. Journal of Urology 156: 517-521, 1996.
12. Bonfil, R.D., Russo, D.M., Schmilovich, A.J., & García-Palazzo, B. Intravesical therapy with Vinorelbine tartrate: antitumor activity in orthotopic murine cell carcinoma of the bladder. Journal of Urology 158: 912-915, 1997.
13. Matar, P., Rozados, V.R., Roggero, E.A., Bonfil, R.D., & Scharovsky, O.G. Modulation of the antimetastatic response against a rat lymphoma by a single-low dose of cyclophosphamide. Tumor Biology 16: 69-76, 1998.
14. Bonfil, R.D., Bustuoabad, O.D., & Binda, M.M. Antiangiogenesis and apoptosis as mediators of concomitant tumor resistance induced by Calu-6, a human lung carcinoma cell line, in nude mice. Oncology Research 10: 15-21,1998.
15. Vinyals, A., Peinado, M.A., Gonzalez-Garrigues, M., Bonfil, R.D., & Fabra, A. Failure of wild type p53 gene therapy in human cancer cell expressing a mutant p53 protein. Gene Therapy 6: 22-33, 1999.
16. Matar, P., Rozados, V.R., Binda, M.M., Roggero, E.A., Bonfil, R.D., & Scharovsky, O.G. Inhibitory effect of lovastatin on spontaneous metastases derived from a rat lymphoma. Clinical and Experimenatl Metastasis 17: 25-32, 1999.
17. Matar, P., Rozados, V. R., González, A. D., Dlugovitzky, D. G., Bonfil, R. D., & Scharovsky, O. G. Mechanism of antimetastatic immunopotentiation by low-dose cyclophosphamide. European Journal of Cancer 36: 1060-1066, 2000.
18. Boquete, A.L., Vargas-Roig, L., López, G.A, Gude, R., Binda, M.M., González, A.D., Ciocca, D.R., & Bonfil, R.D. Differential anthracycline sensitivity in two related human colon carcinoma cell lines expressing similar P-glycoprotein and heat shock proteins 27 and 70 levels. Cancer Letters 165:111-116, 2001.
19. Bonfil, R.D., Gonzalez, A.D., Siguelboim, D., Cuello Carrión, F.D., Ciocca, D.R., Villaronga, A., Metz, L., Mosso, F., Fayad, E., Reale, M., & Schmilovich, A.J. Immunohistochemical analysis of Ki-67, p21Waf1/Cip1 and apoptosis in marker lesions from patients with superficial bladder tumors treated with vinorelbine intravesical therapy in a preliminary phase I trial. British Journal of Urology International 88: 425-431, 2001.
20. Bonfil, R. D., Russo, D. M., Binda, M. M.,Delgado, F. M., Vincenti, M. Higher antitumor activity of Vinflunine than Vinorelbine against an orthotopic murine model of transitional cell carcinoma of the bladder. Urologic Oncology 7: 159-166, 2002.
21. Binda, M. M., Matar, P., González, A. D., Rozados, V. R., Gervasoni, S. I., Scharovsky, O G., Bonfil, R. D. Differential production of angiostatin by concomitant antitumoral resistance-inducing cancer cells. International Journal of Cancer 100: 14-21, 2002.
22. Rotenberg, R.G., Rozas, N.S., Guerri, L., Cher, M.L., Gamboni, M., Lema, N., Bonfil, R. D. Elevated Levels of Angiostatin in Effusions from Patients with Malignant Disease. Oncology Reports 11: 523-528, 2004.
23. Ustach, C.V., Taube, M., Hurst, Jr., N.J., Bhagat, S., Bonfil, R.D., Cher, M.L., Schuger, L., Kim, H-R.C. A potential oncogenic activity of PDGF D in prostate cancer progression. Cancer Research, in press.
24. Scharovsky, O.G., Binda, M.M., Rozados, V.R., Bhagat, S., Cher, M. L., Bonfil, R. D. Angiogenic and antiangiogenic balance regulates concomitant antitumoral resistance. Clinical and Experimental Metastasis, in press.

Office Address: Department of Pathology and Urology
Wayne State University School of Medicine
Scott Hall Room 9105
Detroit, MI 48201
e-mail: dbonfil@med.wayne.edu
office: (313) 577-2879