Services
- Confocal Microscopy, including both point scanning (Zeiss LSM 510, Zeiss LSM 310, Leica TCS SP5) and spinning disk (Perkin Elmer UltraVIEW) technologies
- Multiphoton Microscopy, including Second Harmonic Generation imaging
- Fluorescent Microscopy
- Conventional, widefield epifluorescence
- Optical sectioning using Structured Illumination (via the Zeiss ApoTome system)
- In vivo small animal imaging
- In vivo small animal Positron Emission Tomography (PET) imaging
- In vivo small animal Magnetic Resonance (MR) imaging
- In vivo small animal X-Ray imaging In vivo small animal white light, fluorescence and bioluminescence imaging
- Long-term, live cell imaging. All confocal microscopes are outfitted with live cell imaging chambers to control CO2 flow, temperature and humidity
- Advanced Image Analysis, including 3D and 4D image reconstruction and quantitative measurements
- Preparation and processing of animal and xenograft tissues for histology, including, embedding, cryostat and microtome sectioning, conventional staining, immunohistochemistry, whole mount preparations, in situ Hybridization, histology data analysis, Tunel assay, and Antibody trial.
- Cell analysis for data acquired using the benchtop cytometers and the FACSDiVa. These services range from simple one-color analysis of the cell cycle to multi-color, multi-laser analysis requiring expert knowledge of machine setup and color compensation
- Cell sorting (operator driven), using the FACSDiVa. The DiVa sorts up to 20,000 cells/second in a single or two-way sort or can deposit cells in wells of a variety of microwell plates using its automated cell deposition unit
- Cytometry Data analysis, using any of three offline workstations in the Hudson Webber Cancer Research Center (HWCRC) or one in the Prentis Cancer Research Building that provides access to licensed Cellquest, DiVa and FlowJo software for basic analysis and programs such as Modfit for cell cycle analysis. The researcher can undertake analysis after suitable training, or Core personnel will provide the service.
- Expert consultation, including advide on the choice of application and experimental design
- Training is provided for every new user as well as refresher training for more experienced users, and workshops are provided, as announced
- On site support for use of equipment