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In addition to routine histological and immunocytochemical
equipment, Dr. McAllister's equipment includes: 2 Leitz light
microscopes, a Leitz Macroscope, automated photography system,
stereology program for light microscopy, -80° freezer, paraffin
embedding apparatus, rotary microtome for paraffin sections,
incubator, laminar flow hood, 3 -20o freezers, 3 refrigerators,
liquid scintillation counter, Morris water maze, and a Roto-rod
motor testing system. He also has access to the Phillips 300
electron microscope maintained adjacent to his lab by the
Department of Emergency Medicine.
Most notable for the proposed project is a state-of-the-art
morphometry system ($54,000) coupled to a new Leica DMX epifluorescence
upright microscope ($60,000). The morphometry system is the
most recent version developed by Microbrightfield, Inc. and
includes the following components:
Neurolucida -TM
- 3D neuron reconstruction and analysis
- 3D anatomical brain mapping
- 3D visualization
- Automatic measurement of many parameters
- Serial section reconstruction
- Morphometry
- Interactive image analysis
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Stereo Investigator
- Optical fractionator provides efficient, precise,
unbiased and reliable estimates of the number and
morphometric properties of biological structures
- Allows the operator to work directly with the light
microscope, and with digital or hard-copy images from
confocal microscopes, electron microscopes, and scanning
topographic instruments
- Creates comprehensive maps of cells and anatomical
regions
- Uses either the Lucivid-TM
for direct visualization and quantification through
the microscopy or a video camera with frame grabber
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Hydrocephalus
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