Basic Research at WSU Department of Emergency Medicine
Research Focus and Grants
Our basic science research focuses on the molecular mechanisms of brain injury that occur as a consequence of transient ischemia such as stroke and cardiac arrest and resuscitation. We have made important contributions in areas of lipid peroxidation, activation of the protease calpain during ischemia, the dysfunction of the unfolded protein response, and the inhibition of protein synthesis at the level of translation initiation during reperfusion. In the past 15 years, our laboratories have been supported by the National Institutes of Health ( 5 RO1s, 3 NIH K08s, 1 R55), the Emergency Medicine Foundation (Center of Excellence Award, 5 EMF Research Fellowship Awards), and the American Heart Association (Scientist Development Award, 2 Pre-doctoral Fellowship Awards); all together these research grants total over $6 million.
Basic Research Laboratory Facilities
The laboratory itself occupies 2,200 square feet that includes two office areas for faculty and students. The laboratory is fully equipped for rodent cardiac arrest, resuscitation, and post-resuscitation critical care including a Bausch & Lomb Stereozoom 7 microscope, microsurgical instruments, stereotaxic apparatus, ECG and arterial pressure monitor, infusion pumps, and feedback-controlled heating blanket. We are also equipped for cell culture work with climate-controlled incubators, shakers, and a laminar-flow hood. Major equipment for molecular and structural research includes: Perkin-Elmer 2400 PCR thermocycler, Eppendorf 5804R and 5415C centrifuges, Beckman Advanti J-E refrigerated superspeed centrifuge, LE-80K ultracentrifuge, LS6500 scintillation counter, and DU530 UV-VIS spectrophotometer, BioRad FPLC system and the VersaDoc 4000MP imager with Q-dot filters and quantitation software, -20 and -80 C freezers, AO100 microscopes, Zeiss inverted microscope, Leica DMLB fluorescence microscope with Q-dot filters and cooled CCD digital camera, Neurolucida NL-10/Stereoinvestigator V5.05.1 software, JEOL JEM-1010 transmission electron microscope, LKB Ultratome III, and Leica CM1850 cryostat. For behavioral testing, we have a Morris water maze tank, Sony DCRHC40 digital camcorder, and Biobserve V2.1 software for automated tracking and analysis.
Doctoral Education and Fellowship
The basic research unit offers doctoral education in cooperation with the Department of Physiology. Since 1993, three Emergency Medicine-trained physicians have obtained their Ph.D. through our research fellowship. In addition, we have graduated four Ph.D.s in conjunction with the Department of Physiology. The Emergency Medicine Research Fellowship is identical with the doctoral program in Physiology and accepts applications from emergency physicians who have successfully completed their residency. The Fellowship and doctoral program require 80% time commitment and allow no more than 1 weekly patient care shift in the Emergency Department.
For further information please contact:
Gary Krause, MD, M.S.
Edward S. Thomas Professor
Department of Emergency Medicine
Wayne State Univeristy|
550 East Canfield Ave., 51.2 Lande Bldg
Detroit, MI 48201
gkrause@med.wayne.edu
Introduction to Brain Ischemia Syllabus
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