William S. Brusilow

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Professor Area of interest: ATPase synthesis and assembly, mitochondrial bioenergetics of neurodegenerative diseases, the role of brain glutamine in Huntingtons disease and ALS. Ph.D. University of Wisconsin, Madison, WI, 1980 e-mail: wbrusilo@med.wayne.edu Tel: 313-577-6659

Dr. Bill Brusilow uses E. coli to study the proton ATPase, the enzyme responsible for synthesizing most of the ATP in living systems. His laboratory has addressed many aspects of this enzyme, including the steps in assembly of this multimeric membrane-bound complex, the regulation of expression of the various genes, and how metabolic changes affect subunit stoichiometry and the thermodynamics of energy coupling. A second project, conducted in collaboration with Dr. Needleman, involves studying the anti-proliferative effects of phenylalkanoic acids, using yeast as a model system. These compounds have demonstrated anti-cancer effects in the laboratory and in clinical trials, but the mechanism is unknown. The collaborative yeast studies have demonstrated a number of fundamental effects of these drugs on amino acid uptake, protein turnover, and signal transduction.

Brusilow, W.S.A. Is Huntington's a glutamine storage disease? The Neuroscientist 12, 300-304, 2006.

Grzanowski, A., Needleman, R., Brusilow, W.S. Immunosuppressant-like effects of phenylbutyrate on growth inhibition of Saccharomyces cerevisiae. Curr. Genet. 41:142-149, 2002.

Cao, N.J., Brusilow, W.S,, Tomashek, J.J., Woodbury, D.J. Characterization of reconstituted Fo from wild-type Escherichia coli and identification of two other fluxes co-purifying with Fo. Cell. Biochem. Biophys. 34:305-320, 2001.

Tomashek, J.J., Poposki, J.A., Brusilow, W.S. A functional His-tagged c subunit of the Escherichia coli F-type ATPase/Synthase. Arch. Biochem. Biophys. 387:180-187, 2001.

Matten, S.R., Schneider, T.D., Ringquist, S., Brusilow, W.S. Identification of an intragenic ribosome binding site that affects expression of the uncB gene of the Escherichia coli proton-translocating ATPase (unc) operon. J. Bacteriol. 180:3940-3945, 1998.

Schemidt, R.A., Qu, J., Williams, J.R., Brusilow, W.S. Effects of carbon source on expression of F0 genes and on the stoichiometry of the c subunit in the F1F0 ATPase of Escherichia coli. J. Bacteriol. 180:3205-3208, 1998.

Abu-Shakra, S., Alhalabi, M.S., Nachtman, F.C., Schemidt, R.A., Brusilow, W.S. Anabolic steroids induce injury and apoptosis of differentiated skeletal muscle. J. Neurosci. Res. 47:186-197, 1997.

Schemidt, R.A., Brauning, C.K., Bouvier, A., Brusilow, W.S. Localization of a conformational energy-coupling determinant near the C terminus of the beta subunit of the F1F0-ATPase. J. Biol. Chem. 271:33390-33393, 1996.