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PARAFFIN EMBEDDING
Using the Technicon in Dr. Lamperti's lab, process through
the following:
|
Agent
|
Duration, hrs
|
Time*
|
| |
|
|
| 70% EtOH |
4
|
4-8 PM |
| 80% EtOH |
2
|
8-10 |
| 80% EtOH |
1
|
10-11 |
| 95% EtOH |
2
|
11-1AM |
| 95% EtOH |
1
|
1-2 |
| 100% EtOH |
1
|
2-3 |
| 100% EtOH |
1
|
3-4 |
| Amyl Acetate |
3
|
4-7 |
| Toluene |
1/4
|
7-7:15 |
| Tol:Par(1:1) |
1/2
|
7:15-7:45 |
| Paraffin |
1
|
7:45-8:45 |
| Paraffin |
1
|
8:45-9:45 |
| Embed |
|
|
| |
|
|
* typical schedule for overnight embedding
Embedding should involve ~h or vacuum infiltration followed by embedding
in molds placed on a cold tray (see Phyllis Beeton, 2-~4425for assistance).
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