NISSL STAIN -PARAFFIN SECTIONS (Lamperti)

 

1. Xylene
5 minutes
2. Xylene
5 "
3. 100% EtOH
2-3 "
4. 95% EtOH 2-3 "
5. 70% EtOH 2-3 "
6. 50% EtOH 2-3 "
7. D-H2O 2-3 "
8. Cresyl violet 3-5 "
9. D-H2O 7 dips
10. 70% EtOH 6 "
11. 95% EtOH 5 5 "
12. 95% + Acetic acid Differentiate
13. 95% EtOH Rinse & check
14. 100% EtOH 2-3 minutes
15. Xylene 2-3 "
16. Xylene 2-3 "
Cresyl violet:

cresylechtviolet (Roboz)

2.5 g

sodium acetate

4.1 g

acetic acid

9.8 ml

D-H2O

500.0 ml