Methyl Green Coutnerstaining Procedure

Reagents:

0.8% Methyl Green (Sigma M5015) Sodium Acetate (Sigma S9513)

Recipe:

Dissolve 1.36 grams Sodium Acetate in 80 ml Di H2O. Adjust the pH to 4.0 with Acetic Acid. Add Di H2O to a final volume of 100 ml. Dissolve 0.8 grams Methyl Green in the 100 ml. Filter through a 0.45 or 0.2 micron filter prior to use.

Procedure:

**It should stain normal nuclei a pale to medium green if the pH is correct and the methyl green does not contain any crystal violet
**Not suitable for AEC stained slides