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HRP INCUBATION SOLUTION -TMB (J. Way)
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Solution A:
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Sodium Nitroferricyanide |
100.0 mg
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Acetate buffer, pH 3.3 |
5.0 ml
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D'H20 |
92.5 ml
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___________
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97.5 ml
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Solution B:
97.5 ml 2.5 ml 100.0 ml
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3,3',5,5' Tetramethyl benzidine |
2.5 mg
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100% Ethanol |
2.5 ml
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(Heat to 40°C. to dissolve) |
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Final mixing (seconds before introduction
of sections):
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Solution A |
97.5 ml
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Solution B |
2.5 ml
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__________
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100.0 ml
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