6-HYDROXYDOPAMINE (6-OHDA)

Catecholamine neurotoxin (substantia nigra)

8 micrograms (free base) 6-0HDA (Sigma Chemical Co.) in 4 microliters saline solution containing 0.2 mg/ml ascorbic acid (anti-oxidant)

Inject through 27 gauge needle at rate of 1 ul/min, needle left in place 2 min after finishing