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CRESYL VIOLET -FROZEN SECTIONS (UCLA)
| 1. 95% EtOH |
3-5 min. |
13. D-H2O |
1-3 min. |
| 2. 95% EtOH |
3-5 min. |
14. D-H2O |
1-3 min |
| 3. 100% EtOH |
3-5 min. |
15. 70% EtOH |
1-3 min. |
| 4. 100% EtOH |
3-5 min. |
16. 95% EtOH |
1-3 min. |
| 5. 100% EtOH |
3-5 min. |
17. 95% EtOH |
1-3 min. |
| 6. Chloroform: 100% EtOH, 1:1 |
20 min. |
18. 95% EtOH + Glacial Acetic Acid (add drop- wise to
desired strength) |
1-3 min. if necessary |
| 7. 95% EtOH |
3 min |
19. 100% EtOH |
3-5 min. |
| 8. 95% EtOH |
3 min. |
20. 100% EtOH |
3-5 min. |
| 9. 70% EtOH |
3-5 min. |
21. 100% EtOH |
3-5 min. |
| 10. 50% EtOH (opt.) |
3-5 min. |
22. Xylene |
3-5 min. |
| 11. D-H2O |
2 min. |
23. Xylene |
3-5 min. |
| 12. Stain |
1-10 min. |
24. Coverslip with Permount |
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Cresyl violet acetate:
| Cresyl violet acetate |
1.0 g |
Adjust pH to 3.5 with acetic acid of Na acetate;
filter before each use;
store in dark |
| D-H2O |
1000.0 ml |
| Glacial Acetic Acid |
3.0 ml |
| Sodium Acetate |
0.2 g |
Results: Nissl substance (cytoplasm of soma & proximal dend- rites) =
reddish blue/purple; nucleus = clear; nucleolus = dark blue; axon hillock
= clear.
Cresyl echt violet:
| Cresyl echt violet |
5.0 g |
Filter before each use;
store in dark |
| D-H2O |
1000.0 ml |
| 10% Glacial Acetic Acid |
3.5 ml (50 drops) |
Results: same as Cresyl violet acetate but not as red
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