BOUIN'S SOLUTION

 

Picric acid (saturated aqueous soln., 1.22%)
9.1 g Picric acid in 750 ml. H2O
750.0 ml
Formalin, full strength (37-40% by volume)
250.0 ml
Glacial Acetic Acid
50.0 ml
 
________
 
1050.0 ml

Note: excellent penetration; especially good for young tissue; fix 4-18 hours -no longer; must be washed out of tissue, usually by 10% formalin, or it will interfere with staining.